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AG500 confirmed notable FPase and CMCase actions inside the secondary screening stage; nonetheless, it exhibited really reduced BGL activity when cultivated during the generation medium. It is thought that fungal strains in the genus Trichoderma
Scatter plot representing CMCase exercise utilizing a small-scale assay and the level of secreted protein of different fungal strains based upon a microplate-primarily based screening assay.
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This might be a result of the significant lignin contents of pretreated cassava pulp and occasional silverskin, leading to inhibition on the enzymatic hydrolysis of cellulose [60]. Other pretreatment solutions needs to be applied for additional enhancement on the enzymatic hydrolysis of these types of substrates.
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Then, the possible in the crude enzymes produced by 6 prospect fungal strains for hydrolysis with the pretreated lignocellulosic biomasses was investigated.
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